간단한 설명
Working in a test tube is one thing; working inside a living human cell, with its packed chromosomes and defence systems, is another. This paper showed CRISPR editing human and mouse cells, and doing several edits at once.
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Cong, Ran, Cox, Lin, Barretto, Habib, Hsu, Wu, Jiang, Marraffini and Zhang demonstrated CRISPR-Cas9 genome editing in human and mouse cells, including multiplexed editing at several loci simultaneously and use of a Cas9 nickase to reduce off-target activity.
Sources
- Science · 2013
Multiplex genome engineering using CRISPR/Cas systems ↗