简单说明
Working in a test tube is one thing; working inside a living human cell, with its packed chromosomes and defence systems, is another. This paper showed CRISPR editing human and mouse cells, and doing several edits at once.
深入了解
Cong, Ran, Cox, Lin, Barretto, Habib, Hsu, Wu, Jiang, Marraffini and Zhang demonstrated CRISPR-Cas9 genome editing in human and mouse cells, including multiplexed editing at several loci simultaneously and use of a Cas9 nickase to reduce off-target activity.
Sources
- Science · 2013
Multiplex genome engineering using CRISPR/Cas systems ↗