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Technology · Gene regulation
CRISPRa (gene activation)
Uses a disabled CRISPR protein to switch a gene up rather than to cut it.
Research toolno cuttingresearchregulation
Laboratory researchDemonstrated in the laboratory. There is no treatment in or near the clinic for this use.
Explication simple
Take the CRISPR protein, break its cutting ability, and bolt on a molecular megaphone. It still finds the exact spot you aim it at, but instead of cutting it shouts — telling the cell to make far more of that gene's protein than it otherwise would.
Aller plus loin
CRISPR activation uses catalytically dead Cas9 (dCas9) fused to transcriptional activator domains — VP64, p65, HSF1, or the SunTag and SAM amplification architectures — targeted to a promoter region to increase transcription of the endogenous gene. Because the endogenous locus is used, normal regulation and splicing are preserved, unlike delivering a cDNA copy.
This is the clearest counter-example to "gene editing always means changing DNA" — these tools deliberately leave the sequence alone.
What it is used for
Overwhelmingly a research tool: genome-wide activation screens that ask which genes, when turned up, produce a phenotype of interest. Therapeutically it is attractive for haploinsufficiency — conditions where one working copy of a gene is not enough — because turning up the remaining copy is conceptually simpler than replacing the broken one.
A disabled CRISPR clamp with activator modules driving expression upward. Illustration generated for The CRISPR Atlas — a visual aid, not a photograph or a literal depiction of molecular structure.
Information éducative uniquement
Cette page est une ressource de référence, et non un conseil médical. L'état de la recherche et des autorisations réglementaires évolue ; vérifiez la date de dernière mise à jour ci-dessus et confirmez tout élément important auprès des sources primaires indiquées.
